Coding

Part:BBa_K3428004:Design

Designed by: Alexis Choi, Andrew Sillato, Julia Petreczky   Group: iGEM20_Stony_Brook   (2020-10-26)


COP1(WD40)-VP16 fusion protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 435
    Illegal SapI.rc site found at 376


Design Notes

A truncated version of COP1, which consists of just the WD40 domain, was utilized in order to eliminate the ubiquitin ligase activity.

Note that this part did not undergo real-world characterization due to COVID-19 restrictions.

Source

BBa_K416001, BBa_K1150001

References

[1] Müller, K., Engesser, R., Schulz, S., Steinberg, T., Tomakidi, P., Weber, C. C., Ulm, R., Timmer, J., Zurbriggen, M. D., & Weber, W. (2013). Multi-chromatic control of mammalian gene expression and signaling. Nucleic acids research, 41(12), e124. https://doi.org/10.1093/nar/gkt340